fluorescent probe jc 1 (MedChemExpress)
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MedChemExpress
fluorescent probe jc 1
Fluorescent Probe Jc 1, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 99/100, based on 457 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/fluorescent+probe/JC-1/pmc13483133-262-9-12
Average 99 stars, based on 457 article reviews
Fluorescent Probe Jc 1, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 99/100, based on 457 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/fluorescent+probe/JC-1/pmc13483133-262-9-12
Average 99 stars, based on 457 article reviews
fluorescent probe jc 1 - by Bioz Stars,
2026-10
99/100 stars
Images
1) Product Images from "Carrier‐Free Berberine/Nitidine Chloride Self‐Assembled Nanoparticles Induce Ferroptosis to Overcome Bortezomib Resistance in Multiple Myeloma"
Article Title: Carrier‐Free Berberine/Nitidine Chloride Self‐Assembled Nanoparticles Induce Ferroptosis to Overcome Bortezomib Resistance in Multiple Myeloma
Journal: Advanced Science
doi: 10.1002/advs.77174
Figure Legend Snippet: Berberine (BBR)/nitidine chloride (NC) self‐assembled nanoparticles (BBR/NC‐SAPs) display potent and selective anti‐myeloma activity by inhibiting proliferation, clonogenicity, DNA synthesis, and inducing mitochondrial dysfunction. (A) Multiple myeloma cells (sensitive: 8226, KMS‐11; bortezomib‐resistant: 8226‐BTZR, KMS‐11‐BTZR) and normal B cells (GM12878) were treated for 48 h with a concentration gradient (0, 2, 4, 6, 8, and 10 µg/mL) of free BBR and NC combination (1:1). (B) Multiple myeloma cells (sensitive: 8226, KMS‐11; bortezomib‐resistant: 8226‐BTZR, KMS‐11‐BTZR) and normal B cells (GM12878) were treated for 48 h with a concentration gradient (0, 2, 4, 6, 8, and 10 µg/mL) of BBR/NC‐SAPs. (C) Cells were treated with the indicated concentrations (0, 2, 4, 6, 8, and 10 µg/mL) of BBR/NC‐SAPs. Colony formation assay demonstrating the potent, dose‐dependent inhibition of clonogenic survival. (D) Cells were treated with the indicated concentrations (0, 2, 4, 6, 8, and 10 µg/mL) of BBR/NC‐SAPs. Analysis of DNA synthesis by EdU incorporation assay utilizing flow cytometry. The percentage of EdU‐positive (green) cells decreased in a dose‐dependent manner upon treatment. (E) Assessment of mitochondrial membrane potential by JC‐1 staining employing flow cytometry. Treatment with BBR/NC‐SAPs induced a dose‐dependent loss of mitochondrial membrane potential. Data are presented as the mean ± SD from three independent experiments. Statistical significance was determined by a one‐way ANOVA followed by Dunnett's multiple comparison test versus the control group. * p < 0.05, ** p < 0.01.
Techniques Used: Activity Assay, DNA Synthesis, Concentration Assay, Colony Assay, Inhibition, Flow Cytometry, Membrane, Staining, Comparison, Control
Figure Legend Snippet: NR2F2 is essential for berberine (BBR)/nitidine chloride (NC) self‐assembled nanoparticles (BBR/NC‐SAPs)‐induced ferroptosis in multiple myeloma (MM) cells. (A) Cytotoxicity assays showing that NR2F2‐knockout cells locked the survival threshold and abolished the dosage‐dependent cytotoxicity of BBR/NC‐SAPs as compared to the control. (B) Colony formation assays revealing that NR2F2 knockout preserves clonogenic potential after BBR/NC‐SAPs treatment. (C) Analysis of DNA synthesis by EdU incorporation assay utilizing flow cytometry, demonstrating that NR2F2 knockout preserves cell proliferation potential after BBR/NC‐SAPs treatment. (D) Assessment of mitochondrial membrane potential (ΔΨm) via JC‐1 or similar dye, denoting that NR2F2 deletion prevents membrane depolarization triggered by BBR/NC‐SAPs. (E) Quantification of intracellular Fe 2+ showing blocked iron accumulation in NR2F2‐deficient cells upon BBR/NC‐SAPs treatment. (F) Lipid ROS levels measured by flow cytometry demonstrating that BBR/NC‐SAPs fail to elevate lipid peroxidation in NR2F2‐knockout cells. Ferroptosis‐related protein levels measured by western blot showing that BBR/NC‐SAPs fail to elevate lipid peroxidation in NR2F2‐knockout cells (G, 8226 and 8226‐BTZR; H, KMS‐11 and KMS‐11‐BTZR).
Techniques Used: Knock-Out, Control, DNA Synthesis, Flow Cytometry, Membrane, Western Blot
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